Overview

Introduction sterilizationDefinition and MethodsPhysical Methods - Red HeatHot Air OvenMoist heat and auto clavePasteurizationIR radiationNon IRFiltration5 types of Filtrationchemical method- two types of gasesLiquid stage4 types of Liquid chemicalsApplicationsIntroduction Culture methodsculture media definition and ingredientstypes of mediaphysical mediasemi solid mediasolid mediachemical media 3 typesnon synthetic mediaspecial media 3 typesEnriched mediaselective mediadifferential mediaanaerobic mediaTransport mediaAntibiotic mediaconclusionTwo major contributions to the art of sterilization came in the 1860'swhen the French chemist and microbiologist Louis Pasteurwrote extensively on how germs cause disease and the English physician, Joseph Lister,developed a technique that used carbolic acid as a spray to disinfect instruments and so on. (refer video slides all full info)The first liquid artificial culture medium was created by Louis Pasteur in 1860Previously bacterial growth on daily materials such as some foods had been observedLouis Pasteur was a French chemist and microbiologist renowned for his discoveries of the principles of vaccination microbial fermentation and pasteurizationThe German physician Robert Koch (December 11 1843 - May 27 1910) is considered the father of modern bacteriology for his work demonstrating that specific microbes are responsible for causing specific diseasesKoch discovered the life cycle of the bacteria responsible for anthrax and identified the bacteria that cause tuberculosis and choleraCulture mediaA microbiological culture medium is a substance that encourages the growth support and survival of microorganisms Culture media contains nutrients growth promoting factors energy sources buffer salts minerals metals and gelling agents (for solid media)Different types of culture media are typically divided based on the physical state of the media Liquid culture media commonly called broth Solid and semi-solid culture media commonly called agar. (refer video slides all full info)

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