Overview

Part 1:what is PCR?who invented PCR?How does PCR works?PCR chemical ingredientsPCR DiagramSteps:1 - Denaturation2- Annealing3-ExtensionPart 2:Types of PCRQuantitativeReal time Reverse transcriptionPart 3:MultiplexNestedHigh -fidelityfastheart startGC richLong rangeAP PCRPart 4:application of PCRclinical diagnosis, prenatal diagnosis, retro-viral, bacterial infection, cancer diagnosis, sex determination embryos and forensic medicineStandard ingredients in the mixture are:·the DNA segment of interest·specific primers·heat-resistant DNA polymerase enzyme·the four different types of DNA nucleotides·the salts needed to create a suitable environment for the enzyme to act.Quantitative PCR (qPCR), also called real-time PCR or quantitative real-time PCR, is a PCR-based technique that couples amplification of a target DNA sequence with quantification of the concentration of that DNA species in the reaction.Real-time PCR is the technique of collecting data throughout the PCR process as it occurs, thus combining amplification and detection into a single step. This is achieved using a variety of different fluorescent chemistries that correlate PCR product concentration to fluorescence intensityReverse transcription polymerase chain reaction is a laboratory technique combining reverse transcription of RNA into DNA and amplification of specific DNA targets using polymerase chain reaction. It is primarily used to MEAsure the amount of a specific RNA.Direct detection of microorganisms in patient specimensIdentification of microorganisms grown in cultureDetection of antimicrobial resistanceInvestigation of strain relatedness of a pathogen of interestGenetic fingerprinting (forensic application/paternity testing)Detection of mutation ( investigation of genetic diseases)you can have maximum details in course video

Skills

Reviews