Overview

High Performance Liquid Chromatography (HPLC) techniques are so popular and common in pharma industry, cosmetic industry, chemistry industry, food industry, polymer industry and environment laboratories.High performance liquid chromatography (HPLC) is the crucial and integral analytical tool applied in all stages of drug discovery, development, and commercialization in the pharmaceutical industry, especially. At the each step of drug development, drug substances, ingredients and drug products are controlled and monitored by using HPLC techniques.In this course, chromotography, chromatography principles, types of chromatography, instrumental design of HPLC, HPLC Analysis, Types of HPLC and Descriptors of HPLC have been discussed within all technical details.HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) COURSE SCOPE1 INTRODUCTION 2 INTRODUCTION TO HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) 3 CHROMATOGRAPHY 3_1 History of Chromatography 3_2 What is Chromatography? 3_3 What is Stationary Phase? 3_4 What is Mobile Phase? 3_5 What is Analyte? 4 CHROMATOGRAPHY PRINCIPLES 4_1 Chromatographic Seperation 4_2 Chromatography (Affinity & Interaction) 4_3 Chromatography (Solubility & Adsorption) 4_4 Chromatography (Solubility & Polarity) 4_5 Chromatography (Solubility & Hidrophilic / Hidrophobic) 4_6 Let's Categorize Molecules! 5 TYPES OF CHROMATOGRAPHY 5_1 Chromatography Classification Based on Bed (Column Chromatography, Paper Chromatogrphy, Thin Layer Chromatography) 5_2 Chromatography Classification Based on Separation Mechanism (Affinity Chromatography, Ion Exchange Chromatography, Gel Filtration Chromatography) 5_3 Chromatography Classification Based on Physical States of Phases (Gas Chromatography, Liquid Chromatography) 6 INSTRUMENTAL DESIGN OF HPLC SYSTEM 6_1 Instrumental Design of HPLC System_Simple Technical Design 6_2 Instrumental Design of HPLC System_Technical Design 6_3 Instrumental Design of HPLC System_Equipment Compartments 6_4 Instrumental Design of HPLC System_Solvent Reservoir 6_5 Instrumental Design of HPLC System_Solvent Delivery System (Pump) 6_6 Instrumental Design of HPLC System_Sampler Unit & Injector & Autosampler 6_7 Instrumental Design of HPLC System_Column 6_8 Instrumental Design of HPLC System_Dedector 6_9 Instrumental Design of HPLC System_PC Monitor 7 HPLC ANALYSIS 7_1 HPLC in Analytical Chemistry 7_2 Qualitative Analysis 7_3 Quantitative Analysis 7_4 Quantitative Analysis Example 8 TYPES OF HPLC 8_1 Types of HPLC 8_2 Normal Phase HPLC (NP HPLC) 8_3 Reversed Phase HPLC (RP HPLC) 8_4 Ion Exchange Chromatography (IEX) 8_5 Size Exclusion Chromatography (SEC) 9 DESCRIPTORS OF HPLC & ANALYTICAL METHOD DEVELOPMENT9_1 Retention Time 9_2 Void Volume (Dead Volume) 9_3 Retention Factor 9_4 Selectivity 9_5 Efficiency (Teorical Plate Number) 9_6 Selectivity & Efficiency 9_7 Resolution 10 CONCLUSION

Skills

Reviews