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Who is the father of tissue culture?Gottlieb HaberlandtGottlieb Haberlandt is known as the father of plant tissue culture.Gottlieb Haberlandt (28 November 1854 to 30 January 1945) was an Austrian botanist. His original idea presented in 1902 was called Totipotentiality.ExplantAn explant is the part of a plant which has got the regeneration potential and is capable to give rise to the whole plant.Step 1 Explant for callus cultureStep 2 Surface sterilizationStep 3 Factors affecting callus culture Physical factors Growth regulatorsStep 4 Suspension culture from callusCallus culture callus is the undifferentiated and unorganized mass of plant cellsIt is basically a tumor tissue which usually forms on wounds of differentiated tissues or organsCallus formation in vivo is frequently observed as a result of wounds at edges of stems or rootsExplant for callus culture The explant for callus culture may be the differentiated tissue from any part of the plant The explant for callus culture may be the differentiated tissue from any part of the plant The selected explant tissues may be at different stages of cell division cell proliferation and organization into different distinct specialized structuresIf the explant used possesses meristematic cells then the cell division and multiplication will be rapidFactors affecting callus culture Many factors are known to influence callus formation in invitro culturesMS medium most commonly used physical factors and growth factorsRest will continue with live session.Major types of MediaMS mediaB5 mediaN6 MediaNiches mediaClassification of Plant tissue cultureinorganic nutrientsCarbon Energy sourcesMacro nutrientsMicro nutrientsDifference between macro and micro nutrientsOrganic supplementsVitaminsAmino acidsother organic supplementsactivated charcoalAntibioticsGrowth regulatorsAuxinsCytokininGibberellinsPlant growth promotersPlant growth functionsPlant growth applicationscommercial usageplant growth inhibitorsABAapplications of ABAeffects of ABAEthaline usesSolidifying agentsGelatinPH of MediumSuitable, selection and sterilization of mediumOverview