Whole genome sequencing of bacterial genomes - tools and applications

Go to Course: https://www.coursera.org/learn/wgs-bacteria

Introduction

### Course Review: Whole Genome Sequencing of Bacterial Genomes - Tools and Applications #### Overview The course "Whole Genome Sequencing of Bacterial Genomes - Tools and Applications" available on Coursera dives deep into the cutting-edge technology of Whole Genome Sequencing (WGS) and its pivotal role in modern medicine. As the healthcare sector increasingly relies on WGS for pathogen surveillance and antibiotic resistance management, this course equips participants with essential bioinformatics skills to analyze and interpret complex genomic data. WGS is not just a technological advancement; it's a transformative approach to understanding bacterial genomics. This course uncovers how the integration of WGS into public health initiatives can lead to improved strategies for combating infectious diseases, ultimately influencing global health policies. #### Course Syllabus The course is structured into five comprehensive modules: **Module 1: Introduction to Bacterial Typing and WGS Applications** This module sets the stage by introducing participants to bacterial typing, the significance of WGS, and its applications in monitoring bacterial pathogens and antimicrobial resistance. It's an essential foundation for understanding the stakes involved in modern genomic research. **Module 2: Introduction to Next Generation Sequencing (NGS)** Participants will gain insights into NGS technology, which is the backbone of WGS. This module elucidates the principles of NGS, preparing learners for a more in-depth exploration of the tools available for analysis. **Module 3: WGS Tools for Species Identification and Resistance Gene Detection** This module focuses on practical demonstrations of bioinformatic tools used for species identification and detecting resistance genes. The interactive nature of the demonstrations allows participants to apply theoretical knowledge to real-world scenarios. **Module 4: Tools for Serotyping and Plasmid Analysis** Continuing from Module 3, this section highlights the importance of serotyping in understanding bacterial strains of Salmonella and Escherichia coli. Participants will learn to identify plasmid replicons, key in determining the genetic basis of resistance. **Module 5: Advanced Analytical Tools and Phylogenetic Tree Building** The final module culminates in more sophisticated analyses, including phylogenetics and creating custom databases to identify genetic markers. The summative tutorial exercise reinforces learning, providing an avenue for practical application. #### Recommendations This course is highly recommended for individuals interested in microbiology, bioinformatics, and public health, especially those looking to deepen their understanding of WGS and its applications in fighting antibiotic resistance. **Pros:** - Comprehensive coverage of theoretical and practical aspects of WGS. - Hands-on experience with bioinformatics tools. - Insight into real-world applications and current issues in bacterial genomics. **Cons:** - Requires a basic understanding of genetics and microbiology. - May be intense for beginners without prior experience in bioinformatics. In summary, "Whole Genome Sequencing of Bacterial Genomes - Tools and Applications" is an invaluable resource for both seasoned professionals and newcomers to the field. As WGS technology continues to evolve and play a critical role in public health, this course offers a timely opportunity to enhance your skills and understanding in this essential area of research. Don't miss the chance to equip yourself with the knowledge and tools necessary to make a significant impact in the health sciences.

Syllabus

Module 1

Welcome and introduction to typing of bacteria and use of Whole genome sequencing applied to surveillance of bacterial pathogens and antimicrobial resistance

Introduction to Next Generation sequencing

Whole genome sequencing tools- demonstration of analysis tools for species identification, MLST typing and finding resistance genes

Whole genome sequencing tools- demonstration of analysis tools for Serotyping of Salmonella and Escherichia coli strains , and finding plasmid replicons

Whole genome sequencing tools- demonstration of analysis tools for multiple analyzes, phylogenetic tree building and finding genetic markers from self-made databases and Summative Tutorial exercise